Zeocin博萊霉素 優(yōu)勢產(chǎn)品

 博萊霉素 Zeocin

產(chǎn)品簡介

Zeocin™ 試劑是博來霉素抗生素家族的一個成員。其抗性由編碼一種 13,665 道爾頓蛋白質的 Sh ble 基因賦予。在表達這種蛋白質的細胞中，Zeocin™ 無法結合和切割細胞 DNA。 Zeocin™ 對大多數(shù)好氧細胞有效，可用于選擇哺乳動物和昆蟲細胞系、酵母以及細菌。 選擇細胞時，Zeocin™ 的用量為50-2000 ug/ml（通常 300 ug/ml），具體取決于細胞類型。 

C₅₅H₈₃N₁₉O₂₁S₂Cu 

式量： 1137.41 克/摩爾

Zeocin™ 是 CAYLA 的商標。

ble 抗性基因受一個或多個美國（號 5,021,344 和 5,118,620）以及相關外國保護，Cayla 擁有權，Invitrogen 已從 Cayla 獲得使用許可。 該產(chǎn)品的銷售于研究目的。
　　 
使用:
　　●液體Zeocin可用于細胞培養(yǎng)。 
　　●用于篩選轉化株的Zeocin濃度根據(jù)pH值和鹽濃度改變。 
　　●pH越高，鹽濃度越低Zeocin活性越大。 
　　●普通大腸桿菌菌株篩選可在25＆micro；g／ml zeocin濃度的低鹽LB瓊脂培養(yǎng)基中進行，pH調至7.5。 
　　●動物細胞篩選：根據(jù)不同細胞系從100到750＆micro；g／ml。 
　　●植物細胞篩選：根據(jù)不同的細胞種類和培養(yǎng)基從50到250＆micro；g／ml。 
　　●酵母篩選：取決于種屬和培養(yǎng)基，S。cerevisiaeSh ble轉化株在50＆micro；g／ml濃度的YPD培養(yǎng)基中篩選，pH7.0.304015.

Invitrogen相關資料如下:

Zeocin™ Selection Reagent

Cat. No. R250-01

Description

Zeocin™ Reagent is a member of the bleomycin antibiotic family. Resistanceis conferred by the Sh ble gene which encodes a 13,665 dalton protein. In cellsexpressing the protein, Zeocin™ is prevented frombinding and cleaving the cellular DNA. Zeocin™ iseffective on most aerobic cells and can be used for selection in mammalian andinsect cell lines, yeast, and bacteria. The amount of Zeocin™required for selection will vary between 50-2000 ug/ml (typically300 ug/ml) depending on the cell type.

C55H83N19O21S2Cu

Formula Weight: 1137.41 g/mole

Zeocin™ is a trademark of CAYLA.

The ble resistance gene is the subject ofone or more of U.S. Patent Nos. 5,021,344 and 5,118,620 and foreignequivalents owned by Cayla and licensed to Invitrogen. This product is sold forresearch purposes only. For commercial license information, please contact:Licensing Department, Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, CA92008.

General Specifications

Concentration:       100 mg⁄mL

Brand:       Zeocin™

Form:       Liquid

Product Size:       1 g

ValidatedApplication:       Eukaryotic Selection⁄Stable Cell Line Generation

Bacterial Selection

Contents & Storage

Zeocin™ is provided in solution at a concentration of 100 mg/ml. The 1 gramsize is supplied in 8 vials. The 5 gram size is supplied in one vial. Store Zeocin™ at -20°C inthe dark. Zeocin™ is guaranteed stable for 6 months when properly stored.